Frequency of CTX-M-1 gene in Escherichia coli isolates of ESBL-producing enzyme in clinical samples by polymerase chain reaction (PCR) in Yasuj

Abstract: Introduction and objective: Resistance to β-lactam antibiotics in the clinical isolates, in most cases is caused by β-lactamase enzymes. In recent years, The incidence of broad-spectrum β-lactamase enzymes (ESBLs) among clinical isolates especially E.coli is greatly increased, since the β-lactamase have several subfamilies, using universal primers designed to detect the following complete families could be useful. β-lactamase producing enzymes (ESBLs) of E. coli has created many problems for patients. β-lactamase CTX-M-1 gene is the cause of resistance. The aim of this study was to evaluate CTX-M-1gene in E.coli. Materials & Methods: In this practical study, susceptibility of isolated bacteria to 13 antibiotics were indicated by disk diffusion method according to CLSI guidelines and strains were analyzed for the presence of widespread β-lactamase enzymes via two-disc synergy method. . Thus، The prevalence of CTX-M1 ESBL gene samples were determined using PCR and the data were analyzed using ANOVA. Results: A total of 200 isolates of E.coli were isolated. The presence of CTX-M-1 gene were also isolated using the PCR method.  From 200 strains studied, 62 (31%), of strains produced ESBL. After  PCR processing of 62 produced ESBL, 43 isolates (69.4%)  were identified as CTX-M-1 genes..   Conclusion:The results of this study showed the high percentage of β-lactamase resistance among of E.coli strains. This is a serious public hazard that should be pointed out to measures for preventing this hazard..